Proteomic analysis of Mn-induced resistance to powdery mildew in grapevine.
Identifieur interne : 000620 ( Main/Exploration ); précédent : 000619; suivant : 000621Proteomic analysis of Mn-induced resistance to powdery mildew in grapevine.
Auteurs : Yin An Yao [République populaire de Chine] ; Junru Wang ; Xuemei Ma ; Stanley Lutts ; Chuanchuan Sun ; Jinbiao Ma ; Yongqing Yang ; Varenyam Achal ; Gang XuSource :
- Journal of experimental botany [ 1460-2431 ] ; 2012.
Descripteurs français
- KwdFr :
- Ascomycota (physiologie), Feuilles de plante (microbiologie), Feuilles de plante (métabolisme), Maladies des plantes (microbiologie), Manganèse (métabolisme), Protéines végétales (métabolisme), Protéome (métabolisme), Résistance à la maladie (MeSH), Vitis (microbiologie), Vitis (métabolisme), Électrophorèse bidimensionnelle différentielle sur gel (MeSH).
- MESH :
- microbiologie : Feuilles de plante, Maladies des plantes, Vitis.
- métabolisme : Feuilles de plante, Manganèse, Protéines végétales, Protéome, Vitis.
- physiologie : Ascomycota.
- Résistance à la maladie, Électrophorèse bidimensionnelle différentielle sur gel.
English descriptors
- KwdEn :
- Ascomycota (physiology), Disease Resistance (MeSH), Manganese (metabolism), Plant Diseases (microbiology), Plant Leaves (metabolism), Plant Leaves (microbiology), Plant Proteins (metabolism), Proteome (metabolism), Two-Dimensional Difference Gel Electrophoresis (MeSH), Vitis (metabolism), Vitis (microbiology).
- MESH :
- chemical , metabolism : Manganese, Plant Proteins, Proteome.
- metabolism : Plant Leaves, Vitis.
- microbiology : Plant Diseases, Plant Leaves, Vitis.
- physiology : Ascomycota.
- Disease Resistance, Two-Dimensional Difference Gel Electrophoresis.
Abstract
Previous studies documented that metal hyperaccumulation armours plants with direct defences against pathogens. In the present study, it was found that high leaf Mn concentrations (<2500 µg g(-1)) induced grapevine resistance to powdery mildew [Uncinula necator (Schw.) Burr]. Manganese delayed pathogen spreading after powdery mildew (PM) inoculation, but did not directly inhibit pathogen growth on a long-term basis. It was postulated that the grapevine resistance resulted from the induction of protective mechanisms in planta. To test this hypothesis, the proteome profile was analysed by Difference Gel Electrophoresis (DIGE) methods to identify proteins that are putatively involved in pathogen resistance. A high Mn concentration caused little oxidative pressure in grapevine, but oxidative stress was deeply enhanced by PM stress. Except for a few proteins that were related to oxidative pressure and proteins specially regulated by Mn or PM, most of the detected proteins exhibited similar changes under excess Mn stress and under PM stress, suggesting that similar signalling processes mediate the responses to the two stresses. As well as PM stress, high leaf Mn concentration significantly enhanced salicylic acid concentration and increased the expression of proteins involved in ethylene and jasmonic acid synthesis. The proteins related to pathogen resistance were also enhanced by excess Mn, including a PR-like protein, an NBS-LRR analogue, and a JOSL protein, and this was accompanied by the increased activity of phenylalanine ammonia lyase. It was concluded that high leaf Mn concentration triggered protective mechanisms against pathogens in grapevine.
DOI: 10.1093/jxb/ers175
PubMed: 22936830
Affiliations:
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Chuanchuan" sort="Sun, Chuanchuan" uniqKey="Sun C" first="Chuanchuan" last="Sun">Chuanchuan Sun</name></author><author><name sortKey="Ma, Jinbiao" sort="Ma, Jinbiao" uniqKey="Ma J" first="Jinbiao" last="Ma">Jinbiao Ma</name></author><author><name sortKey="Yang, Yongqing" sort="Yang, Yongqing" uniqKey="Yang Y" first="Yongqing" last="Yang">Yongqing Yang</name></author><author><name sortKey="Achal, Varenyam" sort="Achal, Varenyam" uniqKey="Achal V" first="Varenyam" last="Achal">Varenyam Achal</name></author><author><name sortKey="Xu, Gang" sort="Xu, Gang" uniqKey="Xu G" first="Gang" last="Xu">Gang Xu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22936830</idno><idno type="pmid">22936830</idno><idno type="doi">10.1093/jxb/ers175</idno><idno type="wicri:Area/Main/Corpus">000612</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000612</idno><idno 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last="Ma">Xuemei Ma</name></author><author><name sortKey="Lutts, Stanley" sort="Lutts, Stanley" uniqKey="Lutts S" first="Stanley" last="Lutts">Stanley Lutts</name></author><author><name sortKey="Sun, Chuanchuan" sort="Sun, Chuanchuan" uniqKey="Sun C" first="Chuanchuan" last="Sun">Chuanchuan Sun</name></author><author><name sortKey="Ma, Jinbiao" sort="Ma, Jinbiao" uniqKey="Ma J" first="Jinbiao" last="Ma">Jinbiao Ma</name></author><author><name sortKey="Yang, Yongqing" sort="Yang, Yongqing" uniqKey="Yang Y" first="Yongqing" last="Yang">Yongqing Yang</name></author><author><name sortKey="Achal, Varenyam" sort="Achal, Varenyam" uniqKey="Achal V" first="Varenyam" last="Achal">Varenyam Achal</name></author><author><name sortKey="Xu, Gang" sort="Xu, Gang" uniqKey="Xu G" first="Gang" last="Xu">Gang Xu</name></author></analytic><series><title level="j">Journal of experimental botany</title><idno type="eISSN">1460-2431</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ascomycota (physiology)</term><term>Disease Resistance (MeSH)</term><term>Manganese (metabolism)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (metabolism)</term><term>Plant Leaves (microbiology)</term><term>Plant Proteins (metabolism)</term><term>Proteome (metabolism)</term><term>Two-Dimensional Difference Gel Electrophoresis (MeSH)</term><term>Vitis (metabolism)</term><term>Vitis (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Ascomycota (physiologie)</term><term>Feuilles de plante (microbiologie)</term><term>Feuilles de plante (métabolisme)</term><term>Maladies des plantes (microbiologie)</term><term>Manganèse (métabolisme)</term><term>Protéines végétales (métabolisme)</term><term>Protéome (métabolisme)</term><term>Résistance à la maladie (MeSH)</term><term>Vitis (microbiologie)</term><term>Vitis (métabolisme)</term><term>Électrophorèse bidimensionnelle différentielle sur gel (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Manganese</term><term>Plant Proteins</term><term>Proteome</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Leaves</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Feuilles de plante</term><term>Maladies des plantes</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Plant Leaves</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Feuilles de plante</term><term>Manganèse</term><term>Protéines végétales</term><term>Protéome</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Ascomycota</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Ascomycota</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Disease Resistance</term><term>Two-Dimensional Difference Gel Electrophoresis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Résistance à la maladie</term><term>Électrophorèse bidimensionnelle différentielle sur gel</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Previous studies documented that metal hyperaccumulation armours plants with direct defences against pathogens. In the present study, it was found that high leaf Mn concentrations (<2500 µg g(-1)) induced grapevine resistance to powdery mildew [Uncinula necator (Schw.) Burr]. Manganese delayed pathogen spreading after powdery mildew (PM) inoculation, but did not directly inhibit pathogen growth on a long-term basis. It was postulated that the grapevine resistance resulted from the induction of protective mechanisms in planta. To test this hypothesis, the proteome profile was analysed by Difference Gel Electrophoresis (DIGE) methods to identify proteins that are putatively involved in pathogen resistance. A high Mn concentration caused little oxidative pressure in grapevine, but oxidative stress was deeply enhanced by PM stress. Except for a few proteins that were related to oxidative pressure and proteins specially regulated by Mn or PM, most of the detected proteins exhibited similar changes under excess Mn stress and under PM stress, suggesting that similar signalling processes mediate the responses to the two stresses. As well as PM stress, high leaf Mn concentration significantly enhanced salicylic acid concentration and increased the expression of proteins involved in ethylene and jasmonic acid synthesis. The proteins related to pathogen resistance were also enhanced by excess Mn, including a PR-like protein, an NBS-LRR analogue, and a JOSL protein, and this was accompanied by the increased activity of phenylalanine ammonia lyase. It was concluded that high leaf Mn concentration triggered protective mechanisms against pathogens in grapevine.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22936830</PMID><DateCompleted><Year>2013</Year><Month>01</Month><Day>24</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1460-2431</ISSN><JournalIssue CitedMedium="Internet"><Volume>63</Volume><Issue>14</Issue><PubDate><Year>2012</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J Exp Bot</ISOAbbreviation></Journal><ArticleTitle>Proteomic analysis of Mn-induced resistance to powdery mildew in grapevine.</ArticleTitle><Pagination><MedlinePgn>5155-70</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/jxb/ers175</ELocationID><Abstract><AbstractText>Previous studies documented that metal hyperaccumulation armours plants with direct defences against pathogens. In the present study, it was found that high leaf Mn concentrations (<2500 µg g(-1)) induced grapevine resistance to powdery mildew [Uncinula necator (Schw.) Burr]. Manganese delayed pathogen spreading after powdery mildew (PM) inoculation, but did not directly inhibit pathogen growth on a long-term basis. It was postulated that the grapevine resistance resulted from the induction of protective mechanisms in planta. To test this hypothesis, the proteome profile was analysed by Difference Gel Electrophoresis (DIGE) methods to identify proteins that are putatively involved in pathogen resistance. A high Mn concentration caused little oxidative pressure in grapevine, but oxidative stress was deeply enhanced by PM stress. Except for a few proteins that were related to oxidative pressure and proteins specially regulated by Mn or PM, most of the detected proteins exhibited similar changes under excess Mn stress and under PM stress, suggesting that similar signalling processes mediate the responses to the two stresses. As well as PM stress, high leaf Mn concentration significantly enhanced salicylic acid concentration and increased the expression of proteins involved in ethylene and jasmonic acid synthesis. The proteins related to pathogen resistance were also enhanced by excess Mn, including a PR-like protein, an NBS-LRR analogue, and a JOSL protein, and this was accompanied by the increased activity of phenylalanine ammonia lyase. It was concluded that high leaf Mn concentration triggered protective mechanisms against pathogens in grapevine.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yao</LastName><ForeName>Yin An</ForeName><Initials>YA</Initials><AffiliationInfo><Affiliation>Key Laboratory of Biogeography and Bioresources, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, Xinjiang, PR China 830011. yaoya@ms.xjb.ac.cn</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Junru</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>XueMei</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Lutts</LastName><ForeName>Stanley</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Chuanchuan</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Jinbiao</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Yongqing</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Achal</LastName><ForeName>Varenyam</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Gang</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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PubStatus="entrez"><Year>2012</Year><Month>9</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>9</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>1</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22936830</ArticleId><ArticleId IdType="pii">ers175</ArticleId><ArticleId IdType="doi">10.1093/jxb/ers175</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Achal, Varenyam" sort="Achal, Varenyam" uniqKey="Achal V" first="Varenyam" last="Achal">Varenyam Achal</name><name sortKey="Lutts, Stanley" sort="Lutts, Stanley" uniqKey="Lutts S" first="Stanley" last="Lutts">Stanley Lutts</name><name sortKey="Ma, Jinbiao" sort="Ma, Jinbiao" uniqKey="Ma J" first="Jinbiao" last="Ma">Jinbiao Ma</name><name sortKey="Ma, Xuemei" sort="Ma, Xuemei" uniqKey="Ma X" first="Xuemei" last="Ma">Xuemei Ma</name><name sortKey="Sun, Chuanchuan" sort="Sun, Chuanchuan" uniqKey="Sun C" first="Chuanchuan" last="Sun">Chuanchuan Sun</name><name sortKey="Wang, Junru" sort="Wang, Junru" uniqKey="Wang J" first="Junru" last="Wang">Junru Wang</name><name sortKey="Xu, Gang" sort="Xu, Gang" uniqKey="Xu G" first="Gang" last="Xu">Gang Xu</name><name sortKey="Yang, Yongqing" sort="Yang, Yongqing" uniqKey="Yang Y" first="Yongqing" last="Yang">Yongqing Yang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Yao, Yin An" sort="Yao, Yin An" uniqKey="Yao Y" first="Yin An" last="Yao">Yin An Yao</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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